The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.[6]
Takai S, Long JE, Yamada K, Miki T (1995). "Chromosomal localization of the human ECT2 proto-oncogene to 3q26.1-->q26.2 by somatic cell analysis and fluorescence in situ hybridization". Genomics. 27 (1): 220–2. doi:10.1006/geno.1995.1033. PMID 7665179.
Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M (1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
Kimura K, Tsuji T, Takada Y, Miki T, Narumiya S (2000). "Accumulation of GTP-bound RhoA during cytokinesis and a critical role of ECT2 in this accumulation". J. Biol. Chem. 275 (23): 17233–6. doi:10.1074/jbc.C000212200. PMID 10837491.
Wennerberg K, Ellerbroek SM, Liu RY, Karnoub AE, Burridge K, Der CJ (2002). "RhoG signals in parallel with Rac1 and Cdc42". J. Biol. Chem. 277 (49): 47810–7. doi:10.1074/jbc.M203816200. PMID 12376551.
Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S (2003). "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways". Oncogene. 22 (21): 3307–18. doi:10.1038/sj.onc.1206406. PMID 12761501. S2CID 38880905.
Saito S, Tatsumoto T, Lorenzi MV, Chedid M, Kapoor V, Sakata H, Rubin J, Miki T (2003). "Rho exchange factor ECT2 is induced by growth factors and regulates cytokinesis through the N-terminal cell cycle regulator-related domains". J. Cell. Biochem. 90 (4): 819–36. doi:10.1002/jcb.10688. PMID 14587037. S2CID 1295995.
Hara T, Ishida H, Raziuddin R, Dorkhom S, Kamijo K, Miki T (2004). "Novel kelch-like protein, KLEIP, is involved in actin assembly at cell-cell contact sites of Madin-Darby canine kidney cells". Mol. Biol. Cell. 15 (3): 1172–84. doi:10.1091/mbc.E03-07-0531. PMC363103. PMID 14668487.
Liu XF, Ishida H, Raziuddin R, Miki T (2004). "Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity". Mol. Cell. Biol. 24 (15): 6665–75. doi:10.1128/MCB.24.15.6665-6675.2004. PMC444862. PMID 15254234.
Kim JE, Billadeau DD, Chen J (2005). "The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis". J. Biol. Chem. 280 (7): 5733–9. doi:10.1074/jbc.M409298200. PMID 15545273.
Oceguera-Yanez F, Kimura K, Yasuda S, Higashida C, Kitamura T, Hiraoka Y, Haraguchi T, Narumiya S (2005). "Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis". J. Cell Biol. 168 (2): 221–32. doi:10.1083/jcb.200408085. PMC2171585. PMID 15642749.
Yüce O, Piekny A, Glotzer M (2005). "An ECT2-centralspindlin complex regulates the localization and function of RhoA". J. Cell Biol. 170 (4): 571–82. doi:10.1083/jcb.200501097. PMC2171506. PMID 16103226.
Niiya F, Xie X, Lee KS, Inoue H, Miki T (2005). "Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner". J. Biol. Chem. 280 (43): 36502–9. doi:10.1074/jbc.M508007200. PMID 16118207.
Zhao WM, Fang G (2005). "MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis". Proc. Natl. Acad. Sci. U.S.A. 102 (37): 13158–63. Bibcode:2005PNAS..10213158Z. doi:10.1073/pnas.0504145102. PMC1201590. PMID 16129829.
Hara T, Abe M, Inoue H, Yu LR, Veenstra TD, Kang YH, Lee KS, Miki T (2006). "Cytokinesis regulator ECT2 changes its conformation through phosphorylation at Thr-341 in G2/M phase". Oncogene. 25 (4): 566–78. doi:10.1038/sj.onc.1209078. PMID 16170345.
Niiya F, Tatsumoto T, Lee KS, Miki T (2006). "Phosphorylation of the cytokinesis regulator ECT2 at G2/M phase stimulates association of the mitotic kinase Plk1 and accumulation of GTP-bound RhoA". Oncogene. 25 (6): 827–37. doi:10.1038/sj.onc.1209124. PMID 16247472. S2CID 28079334.
Nishimura Y, Yonemura S (2006). " Centralspindlin ควบคุมการสะสมของ ECT2 และ RhoA ที่บริเวณคอร์เทกซ์บริเวณเส้นศูนย์สูตรในระหว่างการแบ่งเซลล์". J. Cell Sci . 119 (ตอนที่ 1): 104–14 . doi : 10.1242/jcs.02737 . PMID 16352658. S2CID 14865639 .
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases.
Interactions
ECT2 has been shown to interact with PARD6A . [ 7 ]
Further reading
Takai S, Long JE, Yamada K, Miki T (1995). "Chromosomal localization of the human ECT2 proto-oncogene to 3q26.1-->q26.2 by somatic cell analysis and fluorescence in situ hybridization". Genomics . 27 (1): 220– 2. doi :10.1006/geno.1995.1033. PMID 7665179.
